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Genetic Comparison of SEB to LPS for Rapid Diagnostics
Author(s) -
Hiner Mark Charles,
Lehr Rachael,
Jett Marti,
Chakraborty Nabarun
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a911-c
Subject(s) - enterotoxin , microarray , microarray analysis techniques , gene , biology , exotoxin , lipopolysaccharide , microbial toxins , gene expression , toxin , real time polymerase chain reaction , microbiology and biotechnology , immunology , genetics , escherichia coli
Staphylococcal enterotoxin B (SEB), an exotoxin produced by staphylococcal aureus , and lipopolysaccharide (LPS), an endotoxin released from bacterial cell walls, produce similar flu‐like symptoms. By comparing human genetic responses to these toxins, a set of genes can be identified to facilitate rapid diagnosis and potentially prevent lethal shock due to prolonged toxin exposure. SEB‐infected and LPS‐infected RNA samples were gathered from PBMC cells at several time points, between 2 and 72 hours. Gene expression patterns specific for both toxins were determined by microarray analysis and critical genes were identified by GeneSpring analysis software. A set of these genes was selected for analysis based on function, such as apoptosis, cytokine induction, phosphorylation or transporter activity. After designing primers for the target genes, real time PCR and RT‐PCR were used to confirm the gene expression pattern initially established by microarray analysis. The combination of microarray, real‐time PCR and RT‐PCR analysis techniques provides us the opportunity to effectively and accurately identify and confirm a set of toxin‐specific genes. This research is supported by the Pioneer Undergraduate Research Fellowship grant from the University of Wisconsin – Platteville.