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Dynamics of Translation
Author(s) -
Puglisi Joseph D.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a889-c
Subject(s) - ribosome , translation (biology) , transfer rna , computational biology , eukaryotic translation , internal ribosome entry site , protein biosynthesis , ribosomal rna , biophysics , chemistry , biology , rna , biochemistry , messenger rna , gene
Translation is a complex process, involving assembly of the 70S complex at a start codon during initiation, repetitive tRNA selection, peptide bond formation and translocation events during elongation, and termination. To complement the growing structural information on the ribosome, we have used single‐molecule fluorescence methods to observe the dynamics of ribosome and its ligands during translation. We will present an overview of our work that focuses on events during initiation, ribosomal and tRNA dynamics during elongation, and observation of global protein synthesis using single‐molecule methods. The results of this work provide new perspectives on the timescales and molecular nature of dynamics in translation, and the approaches presented here are generally applicable to other complex systems in biology.