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Placental Apoptosis in Normal and Preeclamptic Patients
Author(s) -
Rani Neerja,
Dhingra Renu,
Batla Nirja,
Kumar Rani
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a883-d
Subject(s) - apoptosis , tunel assay , placenta , andrology , preeclampsia , immunohistochemistry , medicine , histopathology , biology , pregnancy , obstetrics , endocrinology , pathology , fetus , biochemistry , genetics
The aim of the study is to demonstrate apoptosis in normal and preeclamptic patients. A total of 20 normal and 20 preeclamptic human full term human placentae were collected from AIIMS labour room immediately after normal vaginal delivery. It was then fixed in 10% neutral buffered formalin and processed for paraffin blocks. 5mm thick sections were cut and mounted on 10% poly‐L‐lysine coated slides. The sections were then subjected for TUNEL technique and the expression of Bcl‐2 was also assessed using immuno histochemistry technique. Apoptotic nuclei were higher in the placentae of preeclamptic patients as compared to normal patients whereas Bcl‐2 expression was low in preeclamptic placentae as compared to normal placentae. Although increased placental apoptosis at term is a normal placental aging process but increased apoptosis along with low expression of Bcl‐2 in preeclamptic patients suggest that the two can influence the histopathology of placenta and its circulation, which in turn may cause intrauterine growth retardation.