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AN IN VITRO DIFFERENTIATION OF HUMAN TOOTH PULP STEM CELLS INTO ODONTOBLASTS WITH TGFβ1, TGFβ3 and BMP‐2
Author(s) -
AlSalihi K. A.,
Suzina A. H.,
Shani A. M.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a873-d
Subject(s) - odontoblast , dentin sialophosphoprotein , chemistry , microbiology and biotechnology , dental pulp stem cells , dentinogenesis , extracellular matrix , bone morphogenetic protein 2 , transforming growth factor , stem cell , bone morphogenetic protein 7 , cellular differentiation , pulp (tooth) , bone morphogenetic protein , in vitro , dentistry , biology , biochemistry , medicine , gene
Transforming growth factor‐β (TGF‐ β) and human bone morphogenetic protein(HBMP) regulate odontoblast differentiation and stimulates dentine extracellular matrix synthesis. This study designed to investigate the effects of TGF‐β1, TGF‐β3 and BMP‐2 on in vitro differentiation of tooth pulp stem cell into odontoblasts. TGF‐β1, TGF‐β3 and BMP‐2 were added to the culture medium in the concentration of 25ng/mL. The differentiated cells showed characteristics features of odontoblast‐like cells in all treatment cultures. The characteristics of these cells were assessed by morphological appearance, expression of the marker dentin sialophosphoprotein (DSPP), increased alkaline phosphatase levels and formation of mineralised nodules in vitro. The results indicate that tooth pulp stem cell is capable of responding and differentiated into odontoblasts in the presence of TGF‐β1, TGF‐β3 and BMP‐2. These factors related to regulation of cell proliferation, odontoblasts cell processes formation, and matrix mineralization.