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Adipocyte accumulation in bone marrow with aging corresponds to a decrease in side population (SP) stem cell numbers and quality
Author(s) -
Sharp John G,
Berger Ann,
Murphy Barbara O'Kane,
Brusnahan Susan,
Jackson John,
Lane Judy,
Garvin Kevin,
Kessinger Anne
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a873-c
Subject(s) - bone marrow , stem cell , bone marrow stem cell , peripheral blood mononuclear cell , population , femur , haematopoiesis , cell , andrology , pathology , medicine , endocrinology , biology , chemistry , surgery , microbiology and biotechnology , biochemistry , in vitro , environmental health
Hematological deficiencies increase with aging and are manifest as anemias, reduced abilities to respond to stress and myelodysplasias. Potentially an underlying mechanism is a decrease in stem cell numbers or quality. This investigation was devised to evaluate side population (SP) stem cells and lower, tip SP (LSP) versus upper SP cells (USP), based on their ability to exclude Hoechst 33342 dye, as a measure of stem cell “quality” with aging. Blood samples and marrow were obtained from the femoral head and trochanteric region of the femur during elective surgery for total hip replacement. Marrow samples were ground using a mortar and pestle and cellularity and lipid content determined. Marrow and blood mononuclear cells were stained with Hoechst dye and the SP profiles acquired. Lipid content was increased in the femoral head as compared to trochanteric marrow and increased with aging. This was confirmed using cadaver material. As marrow lipid content increased, SP cell numbers and the ratio of LSP: USP cells decreased. The quality of blood SP cells was closer to that of trochanteric marrow. This suggests a decline in the ability of the marrow microenvironment to support SP stem cells was associated with an increase in lipid content with aging.