Premium
Dietary fatty acids regulate NPC1L1 gene expression in mouse intestine
Author(s) -
Jesch Elliot D.,
Schuett David M.,
Lee JiYoung,
Weber John S.,
Carr Timothy P.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a861-c
Subject(s) - oleic acid , cholesterol , sterol , linoleic acid , cholic acid , small intestine , food science , chemistry , biology , sunflower oil , fatty acid , biochemistry , stearic acid , organic chemistry
This study was conducted to test the hypothesis that dietary fatty acids regulate the gene expression of the Niemann‐Pick C1 like 1 (NPC1L1) sterol transporter. Male C57BL/6 mice were fed modified versions of the NIH‐07 cereal‐based rodent diet with varying fat sources. The fat sources were palm oil (P), shea butter (S), high oleic sunflower oil (O), safflower oil (L), and menhaden oil (E) to increase the percentage of palmitic, stearic, oleic, linoleic, and EPA/DHA of the diets, respectively. All diets were supplemented with 1.0% cholesterol and 0.5% cholic acid. Total RNA was extracted from the duodenum of each mouse and NPC1L1 mRNA was quantified by real time PCR. Mean plasma total cholesterol levels were 112, 127, 98, 96, and 88 mg/dL in mice fed P, S, O, L and E, respectively. Liver cholesterol, fecal neutral sterol and bile acid output were also quantified. The relative abundance of NPC1L1 mRNA in the small intestine was 1.0, 0.8, 4.8, 6.2 and 4.2 in mice fed P, S, O, L and E, respectively. These data indicate that dietary fatty acids regulate the expression of NPC1L1 and, therefore, could play an important role in intestinal cholesterol absorption. [Supported by NSF‐EPSCoR grant EPS‐0346476 and the Nebraska Agricultural Research Division.]