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Identification by microarray of dietary phosphorus (P) responsive genes in rainbow trout intestine
Author(s) -
Kirchner Severine,
Soteropoulos Patricia,
Sugiura Shozo,
Tian Bin,
Ferraris Ronaldo
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a825-a
Subject(s) - biology , trout , microarray , gene , gene expression , carbonic anhydrase , complementary dna , microarray analysis techniques , rainbow trout , medicine , messenger rna , microbiology and biotechnology , endocrinology , biochemistry , enzyme , fish <actinopterygii> , fishery
The dietary requirement for P has been estimated using growth rates or bone P levels. These conventional indicators of P status however do not detect the early stages of dietary P‐deficiency. We used the 16K Atlantic salmon microarray to detect genes whose expression levels change in trout intestine after 5 d of feeding a low‐P (LP) relative to a sufficient‐P (SP) diet. 46 genes were over‐expressed and 51 were under‐expressed in LP compared to SP fish. Most of them had never been reported to be P‐responsive. The expression of 10% of these genes was then confirmed by real‐time RT‐PCR. We evaluated the adequacy of their use to monitor P status by verifying that their expression levels were highly correlated with plasma P levels. The over‐expression of the carbonic‐anhydrase‐2, one of the key enzymes that maintain pH homeostasis, was found to be a reliable molecular indicator of dietary P‐deficiency. The observed increase of sulfotransferase 2B1 gene expression in P‐deficient fish may be related to a previously described acute alteration of the mRNA levels of cystein‐related genes. Meprin‐1A and nephrosin, two metallo‐endopeptidases from the astacin family whose functions remain unclear, showed, respectively, an over‐ and an under‐expression that were highly correlated with changes in plasma P. We validated the use of the cross‐species hybridization of a salmon cDNA chip with trout RNA and proved that the use of molecular markers to evaluate P‐deficiency was at least as reliable as plasma P evaluation. (USDA NRI 2004 35206 14154 & 2003 3510213520; NSF IBN 0235011)