Effects of Dendroaspis natriuretic peptide on cardiovascular and renal functions in acute hypertensive rabbits

The purpose of this study was to define the effects of Dendroaspis natriuretic peptdie (DNP) on cardiovascular and renal functions in rabbits in acute hypertensive states induced by a infusion of norepinephrine(NE). Urine volume, urinary Na + , K + and Cl − excretions measured during six 20 min collection periods, three prior to and three following DNP treatment. BP increased ~30 mmHg within 5 min of initiation of continuous NE infusion, and then gradually dropped to ~20 mmHg over baseline after 80 min. Within 15 min of treatment with 30 μ/kg DNP, BP was significantly decreased as compared with controls. Intravenous administration of DNP (1, 10, and 30 μ/kg) to rabbits resulted to increase of diuresis and natriuresis, and a decrease in systolic BP. Urine flow, urinary Na + , K + and Cl − excretion, and GFR were increased at different rate. By DNP treatment, plasma and urinary cAMP levels were increased over 2 folds compare with the vehicle. Plasma and urinary cGMP levels were increased over 3 folds in the administration of DNP (10, 30μ/kg). BP, electrolytes, urinary cAMP, and cGMP levels in the administration of DNP (10μ/kg) were greatly higher than that in the administration of DNP (30μ/kg). These results indicate that DNP markedly reduced BP, and increased diuresis and natriuresis in acute hypertensive states, and suggest that DNP has a possibility of its potential use in the treatment of cardiovascular disease states related to catecholamine activation. (supported by a grant of Center for Healthcare Technology Development, Ministry of Education & Human Resources Development)