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Microarray Analysis of Immobilization Stress Triggered Changes in Gene Expression in Rat Adrenal Medulla
Author(s) -
Sabban Esther Louise,
Liu Xiaoping,
Kvetnansky Richard,
Serova Lidia I
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1471-c
Subject(s) - adrenal medulla , gene , biology , egr1 , microarray analysis techniques , microarray , gene expression , transcription factor , junb , microbiology and biotechnology , transcription (linguistics) , genetics , endocrinology , catecholamine , linguistics , philosophy
The adrenal medulla plays a key role in responding to stress. Exposure to immobilization stress (IMO) triggers increased gene transcription and mRNA levels of catecholamine biosynthetic enzymes in rat adrenal medulla, and induces several transcription factors. To obtain a broader picture of genes involved, microarray analysis was applied to RNA from adrenal medulla with “RAE 230A” Affymetrix chip. The GeneTraffic Multi (3.0) and PathwayAssist programs were used for data analysis. A single IMO triggered significant changes (p< 0.05) in 4924 genes with 1770 up‐regulated and 3172 down regulated. About half the genes were undefined. Among the known genes many encoded transcription factors. Some were previously observed, such as Egr1 and c‐Fos. Others, not previously recognized, included ATF3, JunB, CREM, NGF1B. In addition, many genes related to kinase/phosphatase signaling and growth were elevated. Additionally, several genes related to neurosecretion or stress were also up‐regulated. There were also many metabolic and structural related genes identified. The down regulated genes included more undefined genes, with several related to growth or apoptosis, and transcription. Several of observed changes were confirmed by RealTime RT‐PCR, or by immunoblots and immunocytochemisty. The findings reveal that exposure to a single IMO triggers widespread alterations in gene expression.