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Pre‐differentiated embryonic stem cells promote neuronal regeneration by stimulation of cAMP‐dependent pathways.
Author(s) -
Glazova Margarita,
Pak Elena S,
Moretto Justin,
Hollis Sarah,
Brewer Kori L,
Murashov Alexander K
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1460-c
Subject(s) - synapsin i , embryonic stem cell , microbiology and biotechnology , spinal cord injury , stem cell , regeneration (biology) , spinal cord , downregulation and upregulation , synapsin , stimulation , quisqualic acid , neuroscience , neurotrophic factors , biology , glutamate receptor , kainic acid , biochemistry , gene , vesicle , receptor , membrane , synaptic vesicle
We studied contribution of embryonic stem (ES) cells to neuronal recovery after spinal cord injury induced by unilateral microinjections of Quisqualic acid at spinal levels T12‐L2. Following a one‐week period after injury, the pre‐differentiated towards neuronal phenotype ES cells were injected into injury. Expression of neurotrophins and interleukins were studied by immunoassays and real‐time PCR in spinal cord as well in ES cells. The present study demonstrated that pre‐differentiated ES cells express high level of BDNF and IL‐6, and once injected into injured spinal cord supply the host tissue with these active substances. Moreover, ES cell injections increase cAMP level, and enhance expression of synapsin I and cofilin, which play the important roles in axonal growth. Upregulation of cAMP, synapsin I and cofilin indicates that the one of the main pathways initiated by ES cells is cAMP dependent regeneration. This work was supported in parts by grant from North Carolina Biotechnology Center #2004‐MRG‐1104 (A.K.M.)