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Effects of Prenatal Ethanol and Choline on Urinary Vasopressin Excretion in the Rat
Author(s) -
Bird Danielle N.,
Sato Aileen K.,
Pole Ginger L.,
Claybaugh John R.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1444-c
Subject(s) - vasopressin , endocrinology , medicine , offspring , polydipsia , choline , excretion , polyuria , liquid diet , chemistry , ethanol , arginine , pregnancy , biology , biochemistry , diabetes mellitus , amino acid , genetics
Prenatal ethanol (PE) exposure in rats increases water turnover and reduces arginine vasopressin (AVP) synthesis, storage, and release. Supplemental choline (SC) fed to pregnant rats while consuming alcohol normalizes water balance and AVP of offspring. Markers of fetal alcohol exposure are needed for people with mild symptoms. Lowered urinary AVP excretion (U[AVP]V) could provide a noninvasive diagnostic marker and SC should normalize the reduced U[AVP]V. Pregnant rats were fed one of four liquid diets; a control or an ethanol diet (35% of calories from ethanol) from day (D)7–21 of pregnancy with half of the rats fed SC (223 mg/100 ml liquid diet) from D10–21. At 8 weeks of age the offspring were placed in metabolic cages for 4 days. U[AVP]V was measured on D4. Increased water turnover in PE exposed rats was not accompanied by reduced U[AVP]V, but SC nearly doubled the U[AVP]V regardless of the PE exposure, and normalized water turnover. While these results in rats do not support the use of U[AVP]V as marker for PE exposure, they suggest an AVP mediated mechanism of SC correction of the polydipsia and polyuria. Supported in part by a Research Centers in Minority Institutions award, P20 RR11091.