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Gene Expression in Lung: Early Response to Ad Libitum Refeeding after Calorie Restriction in Adult Mice
Author(s) -
Massaro Donald,
Reiland Kristin,
Alexander Emma,
Massaro Gloria DeCarlo,
Clerch Linda Biadasz
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1443-b
Subject(s) - extracellular matrix , regeneration (biology) , biology , microbiology and biotechnology , gene expression , calorie restriction , gene , genetics , endocrinology
Our goal is to identify and manipulate for therapeutic purposes gene expression in lung that is determinative of alveolar regeneration. One obstacle toward this goal is identification of the time of onset of gene expression determinative of alveolar regeneration following an experimental manipulation that induces alveolar regeneration. To identify the onset of signaling at the RNA level that initiates alveolar regeneration, we reasoned to regenerate alveoli requires, for each alveolus, the conversion of a flat component of gas‐exchange surface comprised of epithelium, extracellular matrix, cells within the matrix, and endothelial cells, into a fold (septum). The biological processes involved in forming a septum include cell replication, angiogenesis, cell motion and guidance, and formation of extracellular matrix. Using microarray and RT‐PCR analysis of gene expression in lung, we found evidence at the RNA‐level, of signaling for cell replication and extracellular matrix formation within 3 h of ad libitum refeeding following calorie restriction in mice, a manipulation that induces regeneration of alveoli lost during calorie restriction. We conclude that the first three hours of ad libitum refeeding following calorie restriction is the period in which genes determinative of alveoli regeneration in this model are expressed. Supported by HL20366, HL37666, and HL73558.