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Interaction between airway epithelial cells and peripheral blood immune cells in response to Chlamydia lipopolysaccharide (LPS) challenge
Author(s) -
Tang Xiao Xiao,
Chan Hsiao Chang
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1441
Subject(s) - lipopolysaccharide , cytokine , immune system , microbiology and biotechnology , immunology , biology , cell culture , barrier function , epithelium , monocyte , innate immune system , genetics
The airway epithelia represent the first line of defense against microbile invasion along the respiratory tract. However, the interaction between airway epithelial cells and immune cells in host defense remains largely unknown. A co‐culture system was established to study their interaction in response to Chlamydia LPS challenge. Airway epithelial cell line Calu‐3 with or without peripheral blood lymphocytes and monocytes (PBLM) were grown to confluence and Chlamydia LPS was added to the confluent monolayer to mimic an infectious state. The barrier function and secretory activities of Calu‐3 were measured by the short‐circuit current ( Isc ) technique. Inflammation cytokine array was used to determine whether cytokine signaling is involved in the interaction. Chlamydia LPS challenge resulted in significant reduction in transepithelial resistance (TER) across Calu‐3 monolayers but this effect was greatly attenuated by PBLM in the co‐culture, indicating its modulation of epithelial barrier function. The Isc response to forskolin significantly increased in the co‐culture, suggesting that PBLM could also affect secretory activities of Calu‐3 cells. The array result showed that Calu‐3 in the co‐culture challenged by the LPS can release several cytokines such as Monocyte Chemotactic Protein 1(MCP‐1), indicating that Calu‐3 may interact with PBLM via cytokine signaling; in return, PBLM modulate both barrier function and ion transport of the epithelial cell in host defense against infection. Supported by Strategic Program of The Chinese University of Hong Kong.