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Genetic Analysis of Leukemia Cluster Models
Author(s) -
Fastje Cynthia Darlene,
Cobb Jason,
Witten Mark Lee
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1436-b
We have developed animal exposure models to simulate the metals exposures in the childhood leukemia clusters of Fallon, Nevada and Sierra Vista, Arizona. Female C57BL/6 mice were exposed to either tungsten/cobalt (Fallon) or tungsten/arsenic (Sierra Vista) for one week, mated, and then an additional two weeks of metals exposure. The mouse pups were killed at 4 weeks after birth and the following organs were harvested; liver, spleen, thymus, and bone marrow. Currently, we are examining the role(s) of the following gene translocations; TEL‐AML1, AML1‐ETO, and MLL‐AF4 in these harvested organs. Additionally, we are attempting to examine the role(s) of T‐cell receptor and IgH rearrangements in these metals‐exposed mouse pups. Our goal with this research is to determine whether environmental metal(s) exposures played a role in the development of these childhood leukemia clusters in Fallon and Sierra Vista. Research supported by the Gerber Foundation.