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Group III metabotropic glutamate receptors against elevation of intracellular calcium induced by amyloid β‐peptide (31–35) in cultured cortical neurons
Author(s) -
Zhang Ce,
Zhao Li,
Liu NaiHong,
Zhao ShenTing,
Li LingMin,
Wang Jian,
Qiao JianTian,
Kang* YuMing
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1419-b
Although the mechanisms of amyloid β‐peptide(Aβ) neurotoxicity is still obscure, the disruption of the intracellular Ca 2+ concentration ([Ca 2+ ] i ) homeostasis has been emphasized recently. In the present study, we investigated the effects of L‐SOP, (R,S)‐PPG, the selective agonist of group III mGluRs, coapplying with Aβ31–35 on [Ca 2+ ] i in cultured cortical neurons by real time fluorescence imaging technique using the Ca 2+ ‐sensitive dye Furo‐2/AM. The results showed that: 1. Aβ31–35(5μM, 10μM, 20μM, 25μM) could trigger elevation of [Ca 2+ ] i level in cultured cortical neurons in a dose‐dependent manner; 2. After incubating the cells with L‐SOP(100μM), the elevation of [Ca 2+ ] i induced by Aβ31–35(25μM) could be diminished, showing a reduction rate of 71.94±3.61%; 3. The Aβ31–35(25μM)‐induced elevation of [Ca 2+ ] i was significantly diminished in the presence of (R,S)‐PPG(100μM), which almost completely inhibit the [Ca 2+ ] i elevation induced by Aβ31–35, the reduction rate even up to 89.94±5.76%. Conclusion 1. Aβ31–35 is responsible for [Ca 2+ ] i overloading in cultured cortical neurons in a dose‐dependent manner; 2. Activation of group III mGluRs by novel agonist of L‐SOP, (R,S)‐PPG suppressed the elevation of [Ca 2+ ] i induced by Aβ31–35.