Premium
Cerebrospinal fluid analysis in a spontaneous canine model of demyelinating disease
Author(s) -
Oji Takashi,
Kamishina Hiroaki,
Cheeseman Jennifer A.,
Clemmons Roger M.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1417-d
To evaluate the pathological significance of the cerebrospinal fluid (CSF) in degenerative myelopathy (DM) of the German Shepherd dog (GSD), oligoclonal band pattern, IgG index, and Myelin Basic Protein (MBP) levels were investigated. Commonly recognized in the aged GSD, DM presents as a progressive neurological disorder characterized by the demyelination and axonal loss of the white matter in the spinal cord. The etiology of this disease is still unknown; however, several studies have shown an immune‐mediated pathoetiology. Oligoclonal band pattern was demonstrated by isoelectric focusing with immunoblot detection. The IgG index was calculated by comparing serum and CSF IgG to albumin ratios. In GSD with DM, no significance was revealed in the IgG index, while MBP levels were elevated in CSF of DM patients [4.28 ± 0.89 ng/ml (sem)] and the ratio of MBP/TP was calculated to be increased suggesting release of MBP into CSF of DM patients [0.08 ± 0.02 (sem)]. The result of this study may correspond to the demyelinating disease in human beings as a spontaneous canine model.