Premium
The effects of L‐arginine on the cardiac function of advanced stage of myocardial reperfusion of rats in vivo
Author(s) -
Wang XiaoLiang,
Liang Feng,
Wang Jin,
Tian Jue,
Zhao RongRui,
Ma XinLiang,
Liu* HuiRong
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1410-c
Objective To determine whether nitric oxide may result in deteriorative effects on cardiac function at advanced stage during the course of myocardial ischemia and reperfusion (MI/R) of rats in vivo, and if so, to investigate the mechanisms involved. Methods Thirty‐six male adult rats were randomly divided into three groups, i.e. sham, vehicle and L‐arginine group (n=12). MI/R was produced by ligating the LAD for 30min followed by 5 hours of reperfusion. Rats received vehicle (0.9% NaCl, 1ml/kg, i.v.) or L‐arginine (100mg/kg, i.v.) at 3h after reperfusion. Sham‐operated animals underwent the same surgical procedures except that the suture that was passed under the left coronary artery was not tied. Cardiac hemodynamic parameters and myocardial caspase3 activity were measured. Results Compared with vehicle group, L‐arginine treatment deteriorated the cardiac function(LVSP: 59%±20%vs. 79%±10% at R4.5h, 53%±18%vs. 80%±10% at R5; +dp/dt: 53%±26%vs. 84%±24% at R4.5h, 44%±25% vs. 85%±23% at R5h; −dp/dt: 66%±38%vs. 89%±26% at R4.5h, 54%±33%vs. 90%±24% at R5h. P <0.05), and increased myocardial caspase3 activity (420 vs. 340μmol pNA/mg, P <0.05). Conclusion At advanced stage of reperfusion the cardiac function was deteriorated because of the myocardial apoptosis.