Premium
The distribution of autoantibodies against β 2 ‐adrenoceptor from the sera of patients with heart failure
Author(s) -
zuo lin,
Wang XiaoLiang,
Liu ZhongBao,
He ZhongMei,
Zhao RongRui,
Ma XinLiang,
Liu HuiRong
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1409-c
Synthetic peptides corresponded to amino acids sequences of the second extracellular loop of human β 2 ‐adrenoceptor were used as antigens in a Streptavidin‐ Enzyme‐Linked Immunosorbent Assay (SA‐ELISA) to screen sera from 267 subjects among which 206 had CHF caused by different heart diseases and the remaining 61 healthy donors were as control. The frequency of autoantibodies against β 2 ‐adrenoceptor in CHF group was significantly higher than that in normal control group (NC). The geometric mean titers of autoantibodies against β 2 ‐adrenoceptor in patients with CHF were substantially higher than those in NC (1:89±2.03 vs.1:35±1.79, P<0.01). The autoantibodies decreased the heart rates from 26.43±3.63 to 9.42±3.03, 10.00±1.76, 9.25±2.56. Our results demonstrated that the autoantibodies against β 2 ‐adrenoceptors not only exist in the sera of patients with heart failure resulting from different heart diseases, but also their titers were significantly higher than those in normal control group, and this autoantibody displayed ′agonistic activity′ on the corresponding receptors as evidenced by its decreasing effects on the beating rate of cultured cardiomyocytes.