Premium
Hydrogen peroxide elicits prostaglandins‐mediated constriction of isolated skeletal muscle venules
Author(s) -
Goetschkes Tara,
Toth Janos,
Debreczeni Bela,
Tamas Robert,
Koller Akos
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1400
In several diseases of the cardiovascular system, such as hypertension diabetes mellitus, and hyperhomocysteinemia, there is an increased vascular formation of hydrogen peroxide (H2O2), which could result in functional and morphological changes of vessels. Also, during various surgical procedures H2O2 is used to disinfect tissue surfaces exposing veins and venules (and other tissues) to high concentrations (~ 3% or more) of H2O2, the effects of which are not well characterized. We hypothesized that H2O2 modulates the basal tone of skeletal muscle venules. Thus effects of exogenous H2O2 (10‐7‐ 10‐5 M) on the diameter of isolated, pressurized rat gracilis skeletal muscle venules were investigated. The venules developed spontaneous tone (−16.1±4.2%) in response to an intraluminal pressure of 10 mmHg (passive diameter: 163 ± 15.6 μm, active diameter: 137±7.8 μm, mean±SEM). In a concentration dependent manner (10‐7‐10‐5 M) H2O2 elicited constrictions (max.: from 137±7.8 μm to 61±18 μm, 48.5±5.1%, at 10‐5 M), which were abolished by indomethacin (max.: from 134.6±12.6 μm to 133.4±13.3 μm). Because H2O2 at low micromolar concentrations upregulates the synthesis of constrictor prostaglandins, we suggest that H2O2 could affect to the local regulation of skeletal muscle venular blood flow in various physiological and pathophysiological conditions. (Grants: Hungarian Nat. Sci. Res. Funds, OTKA T‐48376 and NIH USA HL‐46813, AHA NE Aff. 0555897T, APS Summer Research Teacher 2005