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Differences in survival time to controlled hemorrhage among inbred rat strains.
Author(s) -
Klemcke H. G.,
Baer D.,
Cox A.,
Cortez D.,
Garrett M. R.,
Joe B.,
Ryan K. L.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1382-b
Previous observations with outbred rats showed a differential survival to an identical level of hemorrhage (e.g., Torres et. al., Am J Physiol 286:H1811, 2004). In an initial attempt to determine potential genetic components of survival to such hemorrhage, survival time to a controlled hemorrhage was measured in 14 inbred strains of rats. Rats were catheterized, and ~ 24 hours later, 55% of the calculated blood volume was removed during a 26 min period from conscious unrestrained animals. Rats were observed for a maximum of 6 hr. In ongoing studies (n = 3–6/strain) survival time is greatest in Dahl Salt Sensitive rats (SS; 298 ± 62 min; mean ± SEM), when compared with the shortest survival time which occurs in Fisher 344 rats (F344; 46 ± 7 min; P ≤ 0.02). Between these two extremes, survival times for rat strains range as follows: Noble (NBL; 236 ± 76 min); Munich Wistar Fromter (MWF; 216 ± 85 min); Dahl Salt Resistant (SR; 184 ± 91min); Brown Norway (BN; 175 ± 76 min); Copenhagen 2331 (COP; 175 ± 54 min); Spontaneous Hypertensive (SHR; 164 ± 98 min); Lewis (LEW; 143 ± 45 min); Dark Agouti (DA; 92 ± 54 min); Fawn‐Hooded Hypertensive (FHH; 83 ± 27 min); BDIX (BDIX; 78 ± 15 min); ACI (ACI; 54 ± 5 min); and PVG (PVG; 48 ± 7 min). SS rats also have survival times greater (P ≤ 0.02) than those of FHH, BDIX, ACI, and PVG rats. NBL rats survive longer than PVG and F344 rats (P ≤ 0.04). As individuals of an inbred strain are extremely similar genetically, 6.5‐fold differences (SS vs F344) in survival time to hemorrhage suggest genetic components for this complex response.