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Cloning a Putative Zinc Transport Protein from Chalamydomonas reinhardtii
Author(s) -
McBride Fancy T,
Gingrich David J
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1366-a
Cells must regulate the cellular concentrations of metal ions. One aspect of this control involves the use of metal ion transport proteins in membranes and several families of cation transporters have been cataloged. For zinc ions, the zinc transport proteins may be categorized into two major families, the CDS (ZIP) influx proteins and the ZIP efflux proteins. Analysis of Chlaymdomonas genomes has identified several putative metal ion transporters. The overall goal of this project is to clone, express and characterize a putative zinc transporter from the CDS family found in the green algae Chlamydomonas reinhardtii, a unicellular algal model for eukaryotic plant cells. A Chlamydomonas reinhardtii cDNA library was obtained and PCR primers were designed based upon the gene sequence for the putative zinc transporter CrMPT1. An approximately 1600 base pair product has been obtained. Sequence analysis of the PCR product against the Clamydomonas Genome Database sequence indicates agreement with the putative sequence corresponding to a partial (nearly complete) sequence. Further PCR amplifications and analysis to complete the sequence and clone the gene are underway and will be presented. Supported by grants from SUNY Potsdam and State of New York/UUP Professional Development Committee.