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Transcriptional regulation of the human Sia‐α2,3‐Gal‐β1,4‐GlcNAc‐R:alpha2,8‐sialyltransferase (hST8Sia III) during retinoic acid‐induced differentiation of human glioblastoma tumor cell line
Author(s) -
Choi HeeJung,
Kim SeokJo,
Jin UnHo,
Suh SeokJo,
Chung TaeWook,
Lee YoungChoon,
Kang SungKoo,
Kim CheorlHo
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1363-c
In this study, we showed the transcriptional regulation of the human Sia‐alpha2,3‐Gal‐beta1,4‐GlcNAc‐R:alpha2,8‐sialyltransferase (hST8Sia III) during neuronal differentiation of glioblstoma cell line (U‐87MG) induced by retinoic acid (RA), a potent regulator of neuronal cell differentiation. The induction of hST8Sia III by RA is regulated at the transcriptional level in a dose‐ and time‐dependent manner as evidenced by Northern blot and reverse transcription polymerase chain reaction. To elucidate the mechanism underlying the regulation of hST8Sia III gene expression during the differentiation of U‐87MG cells induced by RA, we characterized the promoter region of the hST3Gal hST8Sia III gene. Functional analysis of the 5¡ − ‐flanking region of the hST8Sia III gene by the transient expression method showed that the −1194 to −765 region, which contains a retinoic acid response elements at −992 to −973, functions as the RA‐inducible promoter in U‐87MG cells. In addition, gel shift assays and site‐directed mutagenesis indicated that the CREB binding site at 3143 is crucial for the RA‐induced expression of the hST8Sia III in U‐87MG cells.