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Two Arabidopsis orthologs of the transcriptional coactivator ADA2 have distinct biological functions
Author(s) -
Hark Amy T.,
Vlachonasios Konstantinos E.,
Thomashow Michael F.,
Triezenberg Steven J.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1343-d
Subject(s) - biology , arabidopsis , genetics , gene , coactivator , phenotype , mutant , arabidopsis thaliana , histone acetyltransferase , gene expression , transcription factor
ADA2 is a transcriptional coactivator that associates with the histone acetyltransferase GCN5 in budding yeast as well as in model animal systems. In Arabidopsis , two genes encode proteins that resemble yeast ADA2 and share approximately 50% amino acid sequence identity. Plants harboring a T‐DNA insertion in the ADA2b gene display a dwarf phenotype with developmental defects in several plant organs, especially flowers (Vlachonasios, et al. 2003. Plant Cell 15: 626). Here we describe three separate T‐DNA insertion alleles in the ADA2a gene, which result in no dramatic phenotype when grown under continuous light at 25°C. Both ADA2a and ADA2b are expressed in a variety of plant tissues, suggesting that the distinct phenotypes of ada2a and ada2b mutant plants cannot be ascribed to differences in temporal or spatial expression. Moreover, expression of ADA2a from a constitutive promoter fails to complement the ada2b‐1 mutant phenotype, consistent with the hypothesis that the two proteins have distinct biochemical roles. This research was supported by NSF grant MCB‐0240309, the Michigan State University Foundation, and Muhlenberg College.