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Ultrastructural Analysis of Umbilical Cord Blood Derived Dendritic Cells: Comparative Analysis with Bone Marrow and Peripheral Blood Derived Dendritic Cells
Author(s) -
Myers Erin Marie,
Joshi Deepa,
Grimm Andrew,
Bargar Tom,
Todd Gordon L,
Joshi Shantaram S
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1332-c
Umbilical cord blood (UCB) derived antitumor cytotoxic effector cells are better than peripheral blood or bone marrow derived cells. Dendritic cells (DCs) play a central role in the immunostimulation of cytotoxic T lymphocytes or in augmenting cytotoxicity of lymphokine activated killer (LAK) cells. The precise mechanism of increased antitumor cytotoxicity associated with UCB is not known. In this study we compared the ultrastructure of DCs from cord blood to peripheral blood and bone marrow at various stages of DC maturation using Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). DCs were examined for their nuclear morphology, dendritic processes and endosomal vesicles in the cytoplasm on days 0, 3, 7 and 10 in culture with antigen priming on day 5. Results showed no differences in the nuclear morphology, however, fewer numbers of dendritic processes were seen in UCB‐DCs along with smaller vesicles in UCB‐DCs, compared with more heterogeneous endosomal vesicles in PB‐DCs. There were no other remarkable differences in the ultrastructural morphology in DCs from these different sources. Thus, our results provided the comparative analyses of DCs from cord blood, peripheral blood and bone marrow.