Premium
Genetic characterization of hypertriglyceridemia in diabetic TALLYHO/Jng mice
Author(s) -
Kim Hyoung Yon,
Stewart Taryn P.,
Ritchie Ashley,
Kim Jung Han
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1317-c
The TALLYHO/Jng (TH) mouse strain is a newly established polygenic model for type 2 diabetes characterized by obesity, insulin resistance, hyperglycemia, and an early onset of hypertriglyceridemia. The aim of this study was to identify genetic factors responsible for the hypertriglyceridemia in TH mice. To address this aim, we performed a whole‐genome wide scan using F2 male mice derived from an intercross of F1(C57BL/6J x TH) mice. The F2 mice were fasted for 4 hours, and their plasma triglyceride and total cholesterol levels were measured at 8, 12, 16, and 24 weeks of age. All the F2 mice were also genotyped using 69 simple sequence length polymorphic markers. The phenotype and genotype data were analyzed by quantitative trait loci (QTLs) mapping method using the MapManager QT program. In 150 male F2 mice, a significant evidence for linkage with plasma triglyceride levels was detected on chromosome (Chr) 1 at marker D1Mit113 . This QTL was also significantly linked to plasma total cholesterol levels. For both traits, mice homozygous for TH alleles exhibited higher values than mice heterozygous or homozygous mice for B6 alleles. A suggestive QTL linked to plasma triglyceride levels was also detected on Chr 11. In summary, our genetic study revealed a complex pattern of inheritance and mapped a significant QTL on Chr 1 for hypertriglyceridemia in TH mice. Supported by American Diabetes Association Research Award.