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Effects of Chromium Picolinate and Biotin Supplementation on Insulin Sensitivity and Lipid Profile in Fat‐Fed, Streptozotocin‐Treated Rats
Author(s) -
Sahin Kazim,
Onderci Muhittin,
Tuzcu Mehmet,
Ustundag Bilal,
Cikim Gurkan,
Juturu Vijaya,
Komorowski James R
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1314-b
The present study was designed to evaluate the effects of chromium picolinate (CrPic) and biotin on glucose, cortisol, composite insulin sensitivity index (CISI) and lipids in type 2 diabetes (T2D)‐induced rats. The model of T2D was induced by feeding a high‐fat diet (HFD, 40 % of calories as fat) and then injecting streptozotocin on day 14 (STZ, 40 mg/kg i.p.). The male Sprague‐Dawley rats (n=75, 8 weeks old) were divided into five groups. The control group received a standard diet (12% of calories as fat) while all treatment groups received a HFD for 2 weeks and then were injected with STZ: T1 Group: HFD/STZ; T2 Group: HFD/STZ and 80 mcg CrPic/kg bw.d; T3 Group: HFD/STZ and 300 mcg biotin/kg.d; and the T4 Group: HFD/STZ and 80 mcg CrPic kg bw.d and 300 mcg biotin /kg.d. for 10 weeks. T1 rats had elevated levels of serum glucose, triglycerides (TG), total cholesterol (TC), FFA, SGOT, SGPT and urea compared with the control group (P<0.001). In HFD/STZ‐induced diabetic rats (T4 group), supplemental CrPic and biotin lowered glucose levels (P < 0.001). The CISI in the T4 group significantly increased, whereas cortisol, TC, TG, FFA, SGOT, SGPT and urea levels decreased (P < 0.05). The magnitude of the responses was greatest with the combination of biotin and CrPic over that observed with each nutrient alone. Results of the present study indicate that the combination of dietary chromium (CrPic) and biotin may be beneficial for correcting hyperglycemia and reducing diabetes complications.