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Proteomic Analysis of Shear Stress Protection Mechanisms in Endothelial Cells
Author(s) -
Antczak Julie K,
Greene Andrew S
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1298-c
Previous studies have shown that exposure of endothelial cells to a conditioning period of shear stress elicits a protective response from apoptotic stimuli. Several mechanisms have been proposed for this protective effect although the precise mechanism by which membrane shear interacts with the intrinsic apoptotic pathway remains obscure. In the current study we examined the changes in protein expression in rat vascular endothelial cells (VECs) exposed to TNF‐α (10 ng/ml, 24 hours) following a brief shear conditioning exposure (10 dynes/cm 2 of shear stress for 4 h or 18 h) In order to test the hypothesis that shear stress manipulates the signal transduction pathway of TNF‐α giving overall protection to the cell, we utilized liquid chromatography mass spectrometry (LC/MS) of cytosolic and mitochondrial fractions of the stimulated endothelial cells. LC/MS analysis combined with Western blot confirmation revealed differential activation and localization of the bcl‐2 family of proteins indicating that physiological levels of shear stress initiate a protein sequence that involves signaling to the mitochondria. Proteomic analysis of bcl‐2 proteins and their interacting partners will provide targets for further studies on the role of shear stress in angiogenesis