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An Animal Model of Inflammatory Bowel Disease (IBD) in Stress Conditions
Author(s) -
Shieh KunRuey,
Lee ChiYin,
Kuo Terry B.J.,
Yang ShuChuan
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1271-d
Subject(s) - inflammatory bowel disease , medicine , animal model , stress (linguistics) , disease , linguistics , philosophy
Chronic inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is a multifactorial disease where both genetic and environmental factors are expected to play a role in disease development. In addition to several other risk factors, psychosocial factors are considered to be important for the development or exacerbation of IBD. However, the association between psychological stress and IBD has not been well studied and the findings are not consistent, partly because of the methodological limitations in the previous studies, such as using TNBS or acetic acid to inject into the colon and rectum and induce the symptom of IBD. In this study, we examined whether chronic stress combining the lipopolysaccharide (LPS) can induce the similar IBD¡¦s syndrome in male Sprague‐Dawley rats. After the chronic mild stresses following an acute injection of LPS, body weight and food consumption of rat was decreased, and animal exhibited the anxiety‐like behaviors in both elevated plus maze and open field of behavioral tests and showed diarrhea and hematochezia. Using the myeloperoxidase (MPO) activity and histological examination, the colon and rectum of each rat exhibited IBD‐like syndrome. In conclusion, we successfully established an animal model of IBD in rat under chronic mild stress conditions.