Premium
Characterization of an Experimantal Colitis Model: Bacterial and Chemical Induction
Author(s) -
Jurjus Abdo Romanos,
Hussein Inaya M Abdallah Haj,
Mustapha Mustapha Hussein,
Barada Kassem,
Freund Jean Noel
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1265-b
This study reports a model of inflammation of the bowel maintained for 100 days in rats using a combination of bacteria ( E.coli ) and repeated intracolonic injection of Iodoacetamide. The experiment consisted of 132 adult Sprague‐Dawley rats divided into 4 groups (G). G1 (Sham) consisted of 28 animals injected weekly with vehicle 1% Methyl cellulose (MC) intrarectaly (IR). G2 consisted of 38 animals injected IR weekly with 100 μl iodoacetamide (IA) in 1% MC. On the other hand G3 consisted of 38 animals injected IR with IA and enteropathogenic E.coli independently every week. The last group consisted of 28 animals injected IR only with enteropathogenic E.coli on a weekly basis. The combination of bacteria along with IA could maintain symptoms and signs of weight loss, diarrhea, or loose stools for the whole duration of the experiment, 100 days. Intestinal biopsies showed histological changes consistent of ulcerative colitis and an ongoing inflammatory process as documented by inflammatory cellular infiltrates in lamina propria and morphological changes in the villosities of the colon and selected areas of the small bowel. The myeloperoxidase activity in the mucosa showed 3 fold increase in G3 compared to G1. Further characterization for brush boarder enzymes as well as cytokines involved is being carried out. Supp LNCR