Leukocyte‐migration by carbonic anhydrase in different concentrations of CO2 gas.

It has been known that leukocyte‐migration plays an important role in the inflammation. Carbonic anhydrase (CA), which can control intracellular pH created by the balance between CO2 and HCO3‐ to stimulate the cytokine‐production, is suggested to be a big factor for regulating the inflammation with leukocyte‐migration. The object of this study is to observe changes in leukocyte‐migration in 4 – 8% CO2 gas. After leukocyte was incubated in the medium including CA inhibitor, acetazolamide (AZ; 20, 100, and 500 ug/ml), in different CO2 gas, the distance of the leukocyte‐migration into the filter was measured by a microscope. LPS (0.025 and 0.25 ìg/ml) and IL‐8 (0.2 and 0.4 ìg/ml) were used as stimulators. As the distance of migration increased with LPS, IL‐8, and AZ, dose‐ dependently, the distance in 4 – 6% CO2 gas increased dependently. However, the distance was reduced in CO2 gas, over 6% to 8%, concentration‐dependently. It is concluded that that CO2 gas under 6% could stimulate leukocyte‐migration to estimate CA in cells regulate the leukocyte‐migration hrough the mechanism of pH‐change.