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Aminoguanidine Inhibits Tissue Malondialdehyde (MDA) Levels Induced by Hindlimb Suspension (HLS) in Rats
Author(s) -
Chowdhury Parimal,
Scott Joseph E.,
Soulsby Michael
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1251
Hindlimb unloading by tail suspension in rats is a recognized animal model of simulated weightlessness. In this study we have exposed two groups of rats given either tap water or tap water with 50 mg/L aminoguanidine to simulated microgravity for two weeks. Two other identical groups served as non‐suspended controls. Body weight, food and fluid intakes were monitored at regular intervals. At term, the animals were sacrificed, the following tissues (liver, pancreas, small intestine, kidney, heart, lung, brain and testes) were collected and analyzed for MDA levels and protein concentration. Compared to the nonsuspended control, all tissues in the suspended group showed enhanced levels of MDA (p < 0.05). This response was inhibited in the presence of aminoguanidine (p < 0.05). The fact that the HLS‐induced oxidative biomarker, MDA, is abolished in the tissues from suspended rats by an iNOS inhibitor is indicative of the activation of the nitric oxide synthase pathway in vivo in response to simulated weightlessness (Supported by Arkansas Space Consortium Grant).