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Differential responsiveness to heat shock protein (HSP) induction following heat acclimation
Author(s) -
McClung James P.,
Montain Scott J.,
Cheuvront Samuel N.,
He Juren,
Hasday Jeffrey D.,
Singh Ishwar S.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1247
Subject(s) - acclimatization , heat shock protein , hsp70 , hsp60 , hyperthermia , peripheral blood mononuclear cell , heat shock , shock (circulatory) , biology , microbiology and biotechnology , in vitro , biochemistry , medicine , botany , gene
Heat acclimation abates physiologic stress and improves soldier performance, although the cellular and molecular effects of acclimation are unknown. The present study examined whether acclimation reprograms the heat shock protein (HSP) genes. Peripheral blood mononuclear cells (PBMC) isolated from subjects (n = 8) exposed to a 10‐day heat acclimation program showed marked differences in their HSP response to hyperthermia in vitro . Classical responses to heat acclimation, including reductions in heart rate and core body temperature were observed. The temperature threshold for HSP40 and 70 was unchanged by acclimation, while the threshold for HSP90 induction was reduced from 43° C to 39.5° C following acclimation. The magnitude of HSP40, −70, and −90 expression at 43° C was reduced (48, 27 and 36 % respectively) in post‐ vs. pre‐acclimation cells. HSP60 expression was reduced by exposure to temperatures > 37° C in pre‐acclimation cells, but this effect was attenuated post‐acclimation. These results show, for the first time, that heat acclimation in humans is accompanied by changes in the cellular heat shock response. [supported by MRMC (JPM, SJM, SNC) and NIH and VA Merit grants (ISS and JDH)].