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Study of Rb1 block dysfunction of delayed potassium channel by endothelin‐1 from isolated myocardial cell.
Author(s) -
Zeng Qing hua,
Zhong Guo Gan
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1179-a
Subject(s) - potassium channel , ginseng , potassium , chemistry , endothelin receptor , medicine , endocrinology , patch clamp , collagenase , pharmacology , electrophysiology , biochemistry , pathology , receptor , organic chemistry , alternative medicine , enzyme
Background The main compositions of ginseng include Saponins, Carbohydrates, proteina, polypeptids, Aminoueides and some kinds of Vitamine. Before this, we had stuied Rb1, a monomer of panaxadial, about its character in charmacology and physiology We had determine that Ginseng panaxadiol saponin monomer RB1 possess the protective effect in myocardial ischaemia of raw. Objective The study is to examine if Rb1 block dysfunction of potassium channel by endothelin‐1 from isolated myocardial cell. Metheod Ventricular cells are isolated by collagenase. A standard whole cell delayed potassium current (Ik) is recorded by patch clamp technique. Holding at ¨ C40mV to + 50mV. The current amplitude of Ik is recorded by a whole cell configuration. Results With ET‐1 5nmol/L, Ik current enhances from 1000.35¡À81.83pA to 2000.56¡À85.90 pA(p<0.05) and Ik current is inhibited by TEA (potassium channel blocker). The antagonistic action of Rb1 were observed. Increases Ik current induced by ET‐1 decreases from 2000.56+/− 85.9pA to 828 +/− 81.3pA (p<0.05). Conclusion The result suggest that RB1 possess to block dysfunction of potassium channel by endothelin‐1 inducing.