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Adipose Tissue IL‐18 mRNA and plasma IL‐18 concentration – relation to obesity and HOMA index.
Author(s) -
Leick Lotte,
Berg Dorthe,
Lindegaard Birgitte,
Saltin Bengt,
Pilegaard Henriette
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1171-c
Subject(s) - medicine , endocrinology , insulin resistance , adipose tissue , body mass index , obesity , proinflammatory cytokine , endocrine system , inflammation , hormone
Obesity and a physical inactive lifestyle are associated with an increased risk of developing insulin resistance (IR). This may in part be mediated by increased secretion of proinflammatory cytokines by adipose tissue (AT). To test the hypothesis that obesity and IR are associated with increased AT IL‐18 mRNA expression and that acute exercise and exercise training would regulate AT IL‐18 mRNA expression, female subjects with Body Mass Index (BMI) <30 (n=14) and with BMI>30 (n=6) were examined. Fasting blood samples and biopsies were obtained from the abdominal subcutaneous AT 2h after a standardized breakfast. Additional biopsies and blood samples were obtained at 10min and 2h after an acute exercise bout. After 8 weeks of training, the group with BMI>30 repeated the procedure. The AT IL‐18 mRNA content and plasma IL‐18 concentration were 2 fold higher (p<0.05) in the group with BMI>30 than in the group with BMI<30. AT IL‐18 mRNA expression was positively correlated (r2=0.47, p<0.05) with the surrogate index of insulin resistance HOMA, and correlation between plasma IL‐18‐concentration and HOMA was also evident (r2=0.25, P<0.05). Neither acute exercise nor training had significant effects on AT IL‐18 mRNA or plasma IL‐18. In conclusion, both obesity and IR are associated with elevated AT IL‐18 mRNA and plasma IL‐18 levels supporting that AT acts as an endocrine organ, which is involved in obesity associated complications