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Early renal changes after unilateral ischemia reperfusion (IR) injury.
Author(s) -
Sharma Deepak,
McCarthy Ellen T,
Bhojak Jaya,
Reddy RS,
Savin Virginia J,
Prasad Vidudala VTS,
Sharma Mukut
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1156-b
Subject(s) - proteinuria , albumin , albuminuria , renal function , medicine , creatinine , ischemia , renal ischemia , urine , endocrinology , reabsorption , reperfusion injury , acute kidney injury , kidney , urology
The effects of ischemia‐reperfusion injury (IR) on glomerular filtration barrier are not clear. Recently we have shown that ischemia reperfusion injury increases glomerular albumin permeability (P alb ) in rats. It is not clear whether this injury results in albuminuria/proteinuria. We hypothesized that unilateral renal ischemia‐reperfusion injury results in increased P alb and proteinuria. Methods Male Sprague Daley rats (200–270 g) were used as sham‐treated (abdominal incision) controls or as experimental. Unilateral renal ischemia was induced in experimental rats by ligating left renal artery for 45 minutes followed by reperfusion from 3 to 13 days. P alb was determined using in vitro assay. Urine protein (Up) and creatinine (Uc), total urine protein SDS PAGE, and urine albumin ELISA were carried out. Data were analyzed by unpaired (P alb ) and paired sample t‐test (Up/Uc). Results P alb showed persistent increase after one hour of reperfusion (P alb 1 hr 0.75±0.12; 14 day 0.72±0.20). Proteinuria in experimental rats was not different after 3, 6, 10 and 13 days of reperfusion compared to pre‐injury (p values 0.077, 0.255, 0.057, 0.167 respectively). SDS gel electrophoresis of urinary proteins and ELISA of albumin did not show significant changes as compared to control at 3, 6, 10 and 13 days. Conclusion Increased P alb without proteinuria suggests intact tubular reabsorption of albumin in the early stages of renal injury due to unilateral IR.