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Down‐regulation of cyclin B1 expression by berberine promotes leukemia cells entry into G2/M phase of cell cycle
Author(s) -
Lin ChinChung,
Chung JingGung,
Chen GuangWei,
Kao ShungTe
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1131-c
Subject(s) - berberine , cell cycle checkpoint , cyclin b1 , cyclin dependent kinase 1 , cell cycle , coptis , cyclin , chemistry , coptis chinensis , cyclin b , cell culture , cyclin a , g1 phase , apoptosis , microbiology and biotechnology , cancer research , biology , biochemistry , medicine , genetics , alternative medicine , traditional chinese medicine , pathology
Berberine has a wide range of biological actions that suggest it may be of use in cancer prevention. It was reported previously that berberine induced cell cycle arrest not only at the G0/G1 phase but also at the G2/M phase dependent on the dose. However, the mechanism of berberine‐induced G2/M phase arrest in leukemia has not been fully understood. In the present, the effects of the naturally occurring berberine (the major constituent of Coptis Chinese) on growth, cell cycle, and cyclins A, E, and B1 expression were investigated in the human promyelocytic leukemia HL‐60 cells and murine myelomonocytic leukemia WEHI‐3 cells. The flow cytometry assays indicated that berberine induced cytotoxicity and G2/M arrest in both examined cell lines. Berberine induced G2/M arrest in both examined cell lines were accompanied by increased levels of Wee1 and decreased the Cdc25c, Cdk1 and cyclin B1, but it did not affect CDK2 which were examined by Western blotting assay. Therefore, berberine induced G2/M arrest in both examined cell lines through the inhibition of activation of cyclin B1.