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The Effects of AAC on Erythropoietin Expressions and on blood parameters in Chemotherapeutics‐Induced Anemic Rats.
Author(s) -
Oh Myung Sook,
Chang Mun Seog,
Kim Do Rim,
Kang JiUng,
Kim WonNam,
Kang Soon Ah,
Kim HongYeoul,
Bae Hyunsu,
Park Seong Kyu
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1130-b
Subject(s) - erythropoietin , hematocrit , hemoglobin , medicine , anemia , endocrinology , vitamin b12
AAC is the formula constituted of 2 medicinal herbs, Astragali radix and Angelicae radix (1:1). Erythropoietin (EPO) is a hormone that promotes the formation of red blood cells and initiates the synthesis of hemoglobin, the molecule within red cells. In this study, we investigated the effects of AAC on erythropoietin expressions in rat kidneys and on anemia induced by CYP. Male 4‐week‐old SD rats were divided into four groups and treated following the experimental protocol. After treatment, RT‐PCR was carried out to evaluate the effects of AAC on erythropoietin expressions. Some blood parameters were measured and calculated. Other serum parameters were also measured. The EPO fragment was detected at 365 bp. EPO mRNA levels were decreased in CYP only‐treated group than in the normal, while increased in AAC treated group. FER were 36.3, 30.8, 32.5, and 29.2% in the normal, control, AAC, and EPO groups, respectively. The levels of RBC, WBC, platelet, hematocrit, and Hb in the control group significantly decreased than in the normal, whereas those in AAC or EPO treated group increased than in the control group. Serum iron concentration, TIBC, and vitamin B12 have ameliorated in the AAC‐treated group. In conclusion, AAA has an enhancing effect on EPO expressions at mRNA level in rat kidneys with anemia induced by CYP and AAC could recover chemotherapy derived anemia by improving hematological values and vitamin B12 status.