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Proteome analysis of myocardium from chronic Chagas’ disease patients: identification of immune response, oxidative stress, apoptosis, and metabolism proteins
Author(s) -
Teixeira Priscila Camillo,
Iwai Leo Kei,
Honorato Ronaldo,
Fiorelli Alfredo,
Stolf Noedir,
Kalil Jorge,
CunhaNeto Edecio
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1105-b
Subject(s) - oxidative stress , proteome , peptide mass fingerprinting , immune system , apoptosis , biology , pathogenesis , cardiomyopathy , aconitase , creatine kinase , proteomics , immunology , chemistry , mitochondrion , biochemistry , medicine , heart failure , gene
Chronic Chagas’ disease cardiomyopathy (CCC) is an often fatal outcome of Trypanosoma cruzi infection, showing shorter survival than the clinically similar idiopathic dilated cardiomyopathy (IDC). We used the proteomic approach to obtain the global protein expression profile in CCC heart samples and compare it to IDC samples. Homogenates of left ventricular samples from end‐stage CCC and IDC hearts explanted during heart transplantation were subjected to bidimensional electrophoresis. Protein spots were subjected to tryptic digestion and protein identification was performed by peptide mass fingerprinting. We identified 319 proteins (112 distinct proteins), corresponding mainly to structural and mitochondrial proteins and also proteins related with immune response, apoptosis and oxidative stress processes. Differential CCC/IDC protein expression analysis identified significantly reduced expression of proteins related with the energetic metabolism, such as creatine kinase, aconitase and ATP synthase, in the myocardium of CCC patients, as compared to IDC patients. The shorter survival observed in CCC as compared with IDC could be related to the energetic deficit found in CCC hearts. Furthermore, the identification of a high number of proteins involved with apoptosis, oxidative stress and immune response reinforce the importance of these processes on the pathogenesis of CCC. Supported by: FAPESP and CNPq.