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Characterizing the gene expression pattern of intestinal epithelial cells in inflammatory bowel disease
Author(s) -
Flanagan Ken,
Modrusan Zora,
Cornelius Jennine,
Diehl Lauri
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1094-d
Subject(s) - immune system , inflammatory bowel disease , intestinal epithelium , ulcerative colitis , biology , immunology , intestinal mucosa , epithelium , colitis , antigen , inflammation , gene expression , microarray , disease , gene , pathology , medicine , genetics , biochemistry
The intestinal epithelium serves as a primary interface between the body and the outside world. In addition to forming a physical barrier, intestinal epithelial cells (IEC) actively maintain immunologic tolerance toward the myriad of commensal bacteria as well as food antigens. Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, defines a group of related pathologies characterized by dysregulated immune responses in the intestinal mucosa, and consequent damage to the intestinal epithelium. As a principal mediator of mucosal immunity, it is of interest to discern the gene expression pattern of IEC during development of IBD. Laser capture microscopy was used to dissect IEC from intestinal tissue of SCID mice in a transfer colitis model and an IL10 −/ − model. Microarray analysis of the purified RNA from the dissected IEC revealed a distinct pattern of gene expression suggesting that IEC may be involved in enhancement of infiltration of immune cells, antigen presentation and activation of lymphocytes.