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PHARMACOLOGICAL EFFECT OF GENISTEINE ON THE EXPERIMENTAL FIBROSIS INDUCED IN RATS: ANALYSIS OF HEPATIC STELLATE CELL.
Author(s) -
Rodríguez Lourdes,
Leija Alfonso,
Diaz Tania,
Garrido Germán,
ReyesEsparza Jorge
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1092-a
Subject(s) - hepatic stellate cell , hepatic fibrosis , fibrosis , hepatocyte , chemistry , pathology , medicine , biochemistry , in vitro
Fibrogenesis is initiated by hepatocyte damage that results in the recruitment of inflammatory cells and platelets, and activation of Kupffer cells, with the subsequent release of cytokines and growth factors. Of all liver cells, stellate cells appear to be the primary target of these inflammatory stimuli, for they undergo an activation process during fibrogenesis, and it has been reported that this process plays a key role as the initiating event in hepatic fibrogenesis. Genisteine is a flavonoid that appear to have beneficial on multiple disorder, as cancer, osteoporosis, cardiovascular diseases. The aim of this work was to study the effect of genisteine on experimental fibrosis induced to rats, with particular emphasis on hepatic stellate cell. We used male Wistar rats. We included 4 experimental groups: (1) Control; (2) Genisteína (5 g, v.o.); (3) Fibrosis (CCl4, 4mg/kg, i.p.); (4) Fibrosis + Genisteína. We made a microscopic analysis of liver in order to evaluate the architecture and the presence of collagen. The structural analysis of hepatic stellate cells was made by electron microscopy. As well as we evaluated the liver function. Our results showed that genisteine reduces the amount of collagen in liver (50%) and improved the liver function. The microscopic analysis revealed that tissue from animals treated with genisteine showed a reduction in the number of hepatic stellate cells activated, as well as a reduction in the amount of collagen fibers. Our results showed that genisteine can reduce the fibrosis. Therefore, it can be useful for the treatment of chronic liver disease.