Premium
Selenium metabolism in humans: Response of kinetic pools in plasma to 2 yr of Se supplementation
Author(s) -
Patterson Blossom H.,
Wastney Meryl E.,
Combs Gerald F.,
Patterson Kristine Y.,
Veillon Claude,
Taylor Philip R.,
Levander Orville A.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1069-a
Subject(s) - selenium , pharmacokinetics , urine , chemistry , kinetics , metabolism , excretion , oral dose , medicine , absorption (acoustics) , endocrinology , pharmacology , biochemistry , physics , organic chemistry , quantum mechanics , acoustics
Selenium (Se) is a particularly promising cancer chemopreventive agent. We have been investigating the effects of oral Se supplementation on the metabolism of two forms of Se, selenomethionine (SeMet) and selenite (Sel), by comparing kinetics for 4‐mo before (PK1) and after (PK2) 2 yrs of supplementation with 200 ug of Se as SeMet. For each pharmacokinetic study, thirty free‐living subjects (15 M, 15 F) received 2 oral doses of 2 stable isotope tracers, 150 ug of 74 Se as SeMet and 150 ug of 76 Se as sodium selenite (Sel). A compartmental model requiring ten kinetically distinct compartments in plasma and recirculation of tracer through liver and tissues was developed using data from PK1. The model was then applied to data from PK2. By using the relative absorption of Sel to Se Met and excretion rates of total Se in urine and feces, we were able to combine information on the kinetics of each form and predict the size of each plasma pool in each study. Based on data from 7 subjects, Se‐supplementation increased total plasma Se from 132ng/ml to 252 ng/ml with notable increases in five of the plasma pools. Insights into Se metabolism from this investigation will be crucial to interpreting Se‐intervention results, such as those of the 35,000+ male subject SELECT prostate cancer prevention trial.