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In silico gene regulation study of human selenoproteins.
Author(s) -
Stoytcheva Zoia Raykova,
Douet Vanessa,
Lins August Frederick,
Berry Marla
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1068-b
Subject(s) - selenoprotein , promoter , in silico , transcription factor , selenocysteine , biology , gene , regulation of gene expression , transcriptional regulation , genetics , computational biology , gene expression , oxidative stress , biochemistry , superoxide dismutase , enzyme , glutathione peroxidase , cysteine
At least, 25 human selenoproteins (hSeP) containing selenium as selenocysteine have been identified and most of them are involved in oxidative stress protection. Their promoter activities are of particular interest as many are differentially expressed in specific tissues. In addition, reduced selenoprotein activity can result in a compensatory increase of non‐selenium dependent antioxidants to probably counteract the damaging effect of oxidative stress. These observations suggest that selenoproteins participate in multiple molecular pathways and that their expression may be tightly associated with complex regulatory networks and signaling. Understanding influences on transcription will provide insights into cellular roles of selenoproteins and their connection with the other protective antioxidant pathways. Few selenoprotein promoters have been investigated and exhibited some common transcription factor binding sites. We examine common features of promoters of the 25‐known hSeP by in silico analysis. Collecting transcription factor binding and regulation information will build a unique resource for both cis‐ and trans‐regulatory elements. Our short‐term goal is to provide trans‐regulatory information for hSeP genes of oxidative stress‐related transcription factors. This will give us molecular clues into the complex regulation of hSeP. To confirm quality of the in silico analysis data, we examine promoters of hSeP genes by classical experiments.