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Impact of copper‐status and age on rodent plasma peptidylglycine alpha‐amidating monooxygenase and ceruloplasmin
Author(s) -
Prohaska Joseph R
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1066-a
Subject(s) - ceruloplasmin , lactation , chemistry , medicine , monooxygenase , endocrinology , offspring , enzyme , copper , enzyme assay , gestation , biochemistry , biology , pregnancy , cytochrome p450 , organic chemistry , genetics
Several experiments were conducted to identify a sensitive and improved marker of mammalian copper status during neonatal development. Two plasma cuproenzymes, peptidylglycine alpha‐amidating monooxygenase (PAM) (an enzyme involved in peptide posttranslational activation) and ceruloplasmin (Cp) (a ferroxidase involved in iron mobilization), were compared. PAM was assayed using derivatized peptides and HPLC whereas diamine oxidase activity of Cp was assayed spectrophotometrically. Dietary Cu deficiency (Cu−) was induced in both Holtzman rats and Hsd:ICR (CD‐1) outbred albino mice during late gestation and lactation and compared to Cu sufficiency (Cu+). Pups were sampled at postnatal age 3 (P3), P12, and P28. Dams were sampled at day 21 of lactation. Cu restriction began earlier in rats than mice, embryonic day 7 (E7) compared to E17. Compared to Cu+ pups and dams, Cu− animals displayed biochemical signs consistent with Cu deficiency including lower hemoglobin and liver Cu levels. In Cu+ pups Cp activity rose during lactation whereas PAM activity fell. Reduction in Cp activity was more severe than PAM activity in Cu− offspring and dams. Cp activity was greater in rats than mice whereas PAM activity was similar or slightly greater in mice. Both cuproenzymes changed during neonatal development and when dietary copper was limiting. With proper controls, each enzyme can be used to assess copper status. This research was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number 2001‐00998 and by National Institutes of Health grant HD 39708.