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Beneficial effect of biodegradable polyglycolide (PGA) or polylactide (PLA) polymers on extracellular matrix mineralization on osteoblastic MC3T3‐E1 cells
Author(s) -
Kwun InSook,
Cho YoungEun,
Kim HyeJin,
Jeong Yoonhwa,
Kim YongHa,
Choi JaeWon,
Sung YoungDuk,
Kim YounJung,
Choi SikYoung
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1062
Subject(s) - extracellular matrix , mineralization (soil science) , chemistry , biodegradable polymer , extracellular , biomedical engineering , biomaterial , polymer , biochemistry , organic chemistry , medicine , nitrogen
Porous matrices of biodegradable polymers, such as polyglycolide (PGA) or polylactide (PLA) can be used as scaffolds in bone tissue growth during bone repair process. These polymers are highly porous and serve as a template for the growth and organization of new bone tissue. We evaluated the effect of PGA and PLA polymers on osteoblastic MC3T3‐E1 cell extracellular mineralization. MC3T3‐E1 cells were cultured in one of the media containing 4 different biodegradable polymers (normal differentiation medium, PGA plate, PGA plate, PGA/fetal bovine serum(FBS) plate, and FBS plate) and examined the cell morphology. Cell layer was stained using Alizarin red stain for Ca deposit in extracellular matrix. During the culture, PGA plate was resorbed more efficiently than the PLA plate. On clarifying the PGA effect of binding medium or cellular Ca, PGA plate might hold osteoblasts within the plate placement and can be beneficial on the bone formation. PGA and PGA/FBS plate treatment showed better Ca deposits than other treatment although PLA plate treatment also showed reasonable Ca deposit. Cellular morphology showed that PGA/FBS plate treatment showed the most well‐differentiated. The results suggest that PGA or PLA plate can be used as a biomaterial for osteoblastic extracellular matrix mineralization. (This work was supported by RIS project 2004‐2007, Korea Ministry of Industry)