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Promotion of bone growth by a novel extract of fermented soybean germs (AglyMax) in ovariectomized mice fed a high‐fat diet
Author(s) -
Wang Changzheng,
Huang Lingyu,
pan Weijun,
Zhou Jinrong
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1061-b
Subject(s) - ovariectomized rat , daidzein , glycitein , chemistry , genistein , isoflavones , osteoporosis , bone density , medicine , endocrinology , bone mineral , food science , biochemistry , hormone
Daidzein is one of the most abundant isoflavones present in soy and it is unique as it can be further metabolized to equol, a compound with greater estrogenic activity than other isoflavones. The aglycone forms of daidzein may be more potent biologically than its conjugated form. Our preliminary study showed that a novel extract of fermented soybean germs (AglyMax) that is rich in daidzein aglycones promoted bone growth in male mice. Seventy percent of the extract by weight was isoflavones with daidzein:genistein:glycitein aglycones in the ratio of 7:1:2. The objective of this study was to determine the effects of AglyMax on bone in ovariectomized mice. The mice (6 weeks old) were randomly assigned into the treatment groups. A baseline group was killed at the beginning of the experiment. Group A was sham‐operated but fed the high‐fat diet with 60% of the energy from fat; Groups B, C, and D were all ovariectomized and fed the high fat diet containing 0, 1.2 g or 5 g of AglyMax per kg diet, respectively. The feed intake was maintained at similar levels during the experimental period. At the end of the 12‐week experiment, femur bones were analyzed for bone mineral content and density by dual‐energy X‐ray densitometry (Lunar DPX‐IQ). Each bone was also evaluated by a three‐point bending test for its breaking strength. Groups A and D had significantly higher bone mineral content, bone density, breaking strength, bone ash and calcium content than Groups B and C (all at P¡Ü0.01). The baseline group was lowest in all the parameters measured. These results suggest that AglyMax at 0.5% of the high fat diet promoted bone growth in ovariectomized mice.