Premium
Anti‐tumor mechanisms of orally administered a fungal immunomodulatory protein from Flammulina velutipes in mice
Author(s) -
Chang HuiHsin,
Sheu Fuu
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1057-b
Subject(s) - flammulina , immune system , mhc class i , cancer research , in vivo , oral administration , antibody , spleen , biology , immunology , medicine , major histocompatibility complex , pharmacology , mushroom , food science , microbiology and biotechnology
FIP‐ fve , a fungal immunomodulatory protein that exhibited the potential effects on modulating immune responses including anti‐tumor activity, was isolated from the fruiting bodies of the edible mushroom, Flammulina velutipes . The objectives of our present study were to investigate the anti‐tumor effect of FIP‐ fve and its related mechanisms. Oral administration of FIP‐ fve significantly increased the life span and inhibited the tumor size of BNL 1MEA.7R.1 hepatoma‐bearing mice. Oral administration of FIP‐ fve could also enhance the tumoricidal abilities of spleen cells and peritoneal cells obtained from FIP‐ fve fed mice. However, injecting anti‐IFN‐γ antibodies inhibited the effect of FIP‐ fve suppressing tumor formation and tumoricidal activities. In addition, oral administration of FIP‐ fve could enhance the expression of MHC class‐I and MHC class‐II in peripheral blood monocytes and significantly decrease the BrdU uptaking of tumor cells and CD31 expression of tumor tissue. Taken together, oral administration of FIP‐ fve displays anti‐tumor activities in vivo associated with activating both non‐specific and specific immunity of its host and IFN‐γ is demonstrated to be the key point of anti‐tumor activity of FIP‐ fve .