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EFFECTS OF DIETARY CYCLOXYGENASE INHIBITORS INDOMETHACIN AND ASPIRIN ON EGG YOLK ANTIBODY DEPOSITION
Author(s) -
Trott David L,
Butz Dan E,
Cook Mark E
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1055-c
Subject(s) - yolk , adjuvant , immune system , antibody , aspirin , lipopolysaccharide , cyclooxygenase , antibody titer , titer , chemistry , pharmacology , andrology , medicine , immunology , biology , enzyme , biochemistry , food science
Prostaglandins (PG) synthesized from cyclooxygenase (COX)‐dependent pathways have known immune modulating effects in mammals, but are not well studied in avian species. Previous research shows E. coli bacterin used as an adjuvant suppresses egg antibody production possibly by lipopolysaccharide endotoxin stimulating COX. The effect of dietary COX inhibitors on egg antibody deposition was examined in a 3x2 factorial design with 3 diets and 2 adjuvants. SCWL hens were injected with 3mg/ml phospholipase A 2 in either a 1:2 mixture of PBS and complete Freund's adjuvant (CFA) or a 1:2 mixture of E.coli bacterin and CFA. Diet consisting of breeder's mash containing no COX inhibitor, 50 mg/kg indomethacin, or 1 g/kg aspirin was fed throughout the trial. ELISA was used to measure antibody titer in weekly egg yolk samples from day 21 to day 56 after immunization. E. coli adjuvant suppressed titer to 46% of control eggs (p<0.01), but suppression was reversed by addition of COX inhibitors to diet (p<0.01). Titer of indomethacin‐fed hens was 1.6X higher than control hens on days 35–56 after immunization (p<0.10), and titer of aspirin‐fed hens was 3.3X higher (p<0.001). Immune enhancing effects of dietary COX inhibitors were not observed until day 35 which suggests that PGs may play a role in attenuation of immune response.