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Long chain triglyceride (LCT)‐based lipid emulsions increase and olive oil(OO)‐based lipid emulsions decrease leukocyte invasion and mortality in a model of acute lung injury
Author(s) -
Mayer Konstantin,
Bi Ming Hua,
Schaefer Martina B.,
Wang B. E.,
Seeger Werner
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1055-b
Subject(s) - arachidonic acid , triglyceride , chemistry , emulsion , lung , lipid droplet , fat emulsion , inflammation , olive oil , medicine , endocrinology , immunology , biochemistry , cholesterol , food science , enzyme , parenteral nutrition
Acute lung injury (ALI) is initiated by increased alveolar MIP‐2 and subsequent leukocyte invasion. Arachidonic acid (AA) gives rise to eicosanoids and through these controls inflammation. LCT‐based lipid emulsions used may also provide precursors for lipid mediators. We compared impact of an olive oil‐based (OO) vs. a LCT‐based lipid emulsion on ALI in a murine model. Mice were infused for three days with LCT, OO, or NaCl, using a changeable osmotic pump and a central venous line. ALI was induced by endotoxin (LPS) instillation intra‐tracheally. Infusion of OO significantly reduced free AA compared to LCT. After LPS challenge, MIP‐2 markedly increased at 4h, and decreased at 24 h in all groups. However, it was massively increased at 8h in the LCT group in contrast to the OO and NaCl groups. LPS induced a marked recruitment of leukocytes into the lung peaking at 8h in NaCl group. Leukocytes continued to rise in the LCT group in contrast to the OO and NaCl groups. Mortality assessed at 24 h was 0 % and 20% in the NaCl and OO groups, respectively, but increased to 80 % in animals receiving LCT. In a murine model of ALI, infusion of LCT increased free AA, amplified rise in MIP‐2, induced a prolonged recruitment of leukocytes into the lung, and increased mortality. Infusion of LCT‐based lipid emulsions may aggravate ALI but OO‐based lipid possibly offer an alternative choice with a more beneficial profile. Supported by DFG, SFB 547