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Polyunsaturated Fatty Acids Decrease Poly (I:C)‐Induced IL‐8 Production in Caco‐2 Cells
Author(s) -
Li Nan,
Lopez Mariela M,
Neu Josef
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1055-a
Subject(s) - polyunsaturated fatty acid , docosahexaenoic acid , linoleic acid , caco 2 , chemistry , eicosapentaenoic acid , inflammation , biochemistry , fatty acid , biology , immunology , in vitro
Polyriboinosinicpolyribocytidylic acid [poly (I:C)] is a synthetic double‐stranded RNA that is used experimentally to model viral infections. It has recently been identified as one of the critical stimuli for toll like receptor3 (TLR3). Previous studies have demonstrated that polyunsaturated fatty acids (PUFA), especially n‐3 PUFA, have anti‐inflammatory properties. However, the effects of n‐3 PUFA on viral induced inflammation in intestinal epithelia have not beenreported. We hypothesized that n‐3 not n‐6 PUFA will inhibit poly (I:C) induced IL‐8 production in Caco‐2 intestinal cells. Docosahexaenoic acid (DHA, 22:6 n‐3) was used as n‐3 PUFA and linoleic acid (LA, 18:2 n‐6) as n‐6 PUFA. Differentiated Caco‐2 cells were pretreated with 100μM DHA or LA for 24 hours. The cells were then treated with poly (I:C) 50μg/ml for another 24 hours. The culture medium was collected to measure the IL‐8 production by ELISA. Poly (I:C) increased IL‐8 production by 3 fold (p<0.01). DHA and LA deceased poly (I:C)‐induced IL‐8 production by 75% and 40%, respectively. These results indicate that both n‐3 and n‐6 PUFA inhibit poly (I:C) induced inflammatory response, suggesting that PUFA may play an important role in the anti‐inflammatory response caused by viral infection in intestinal epithelia.