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The fast, soluble milk protein stimulate the splanchnic, but not the peripheral metabolism of meal nitrogen as compared to casein in humans
Author(s) -
Fouillet Helene,
Lacroix Magali,
Gaudichon Claire,
Bos Cecile,
Juillet Barbara,
Benamouzig Robert,
Tomé Daniel
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a1045-d
Subject(s) - postprandial , anabolism , chemistry , catabolism , casein , splanchnic , ingestion , meal , medicine , metabolism , endocrinology , carboxypeptidase , food science , biochemistry , biology , insulin , enzyme , blood flow
In the postprandial phase, soluble milk protein are more oxidized than casein due to their faster delivery from the gut, but the other impacts on the splanchnic and peripheral metabolism of a fast vs slow amino acid supply remain unclear. Clinical experiment was coupled with compartmental modeling in order to investigate the postprandial utilization of three 15 N‐labeled milk products: total milk protein (microfiltered milk, MF), micellar caseins (CAS) and milk soluble protein isolate (MSPI). Experimental data for the meal nitrogen (N m ) postprandial kinetics in blood and urine were obtained in healthy adults fed a single meal containing MSPI, CAS or MF after a prior 7d standardization of the diet (1.3 g.kg −1 .d −1 of protein). These data were then analyzed using a 12‐compartment model that allowed to predict the cascade of N m ingestion, absorption, splanchnic uptake for both catabolic and anabolic purposes and further peripheral availability and anabolic use. As shown in the table above, compared to MF, CAS was slow and WPSI was fast according to the rate of their absorption. The fastest protein (WPSI) stimulated both the oxidative and anabolic metabolic pathways in the splanchnic bed, while the slowest protein (CAS) induced the highest peripheral protein accretion.